Recent technological advances by RayBiotech have enabled the largest commercially available glycosylation antibody array to date. With the Human Glycosylation Array L4, researchers can now efficiently obtain glycosylation profiles in their samples. The glycosylation profiles of 500 human proteins can be simultaneously detected, including, but not limited to, cytokines, chemokines, adipokines, growth factors, angiogenic factors, proteases, soluble receptors, soluble adhesion molecules in cell culture supernatants, serum, plasma and other samples types.

Capture antibodies are printed onto glass slides and the glycans on these capture antibodies are removed. The glass slide arrays come pre-blocked and are ready to be incubated with samples. After incubation with samples and washing to remove unbound proteins, five unique biotin-labeled lectins are incubated with the array to allow binding to the glycans from captured target proteins. Streptavidin-conjugated fluorescent dye (Cy3 equivalent) is then added to the array. Finally, the glass slide is dried and laser fluorescence scanning is used to visualize the signals. These signals are then compared to the array map to identify glycosylated proteins present in the samples.

HSPA1A, HTRA1, Human Agrin, IBP160 , IDH1, IDH3A, IFRD1 , IMP2, IGFBP-rp1, IGSF4B, Ihh, ILK, Inhibin beta, Integrin b1, Integrin beta 6, Intergrin a6, IQGAP1 , IQGAP2 , IRE1, IRS2, ISOC2, ITGB4BP, ITIH1, ITIH2, ITIH3, ITIH4 a, JAM-A, JARID2, Karyopherin beta 1, Keratin 36, Keratin 38, KHSRP, KIAA0319L, KIAA1468, KIAA1967, KIF5B , Kilon, KLK-B1, KMD4B, KMT2B, KRT31, KRT72, KRT73, KRT82, KRT85 N-terminal, KRTDAP, KRTHA3B, KSR1, LAD, LAF4, LAIR1, LAM b1, LAMA, Lamin A/C, Lamin B1, Lamin B2, Laminin 2 alpha, Laminin b2, Laminin gamma 1, LAMP, LAMP1, LAMP2, LAP3, LASP1, Latent TGF beta bp2, LCAT, LCMT2, LDHA, LDHB, LEDGF, LEKTI, LILRA3, LIMS1, LMAN2, LMW-PTP, LOK, LOX, LOXL1, LRP 4, LTA4H, LTBP4, Lubricin, LUZP1, LYPA1, Lysozyme, MAGI2, MAGP-2, MAN1, MANF, Mannosidase II, MAP1A, MAPRE1, MARCKS, Complement MASP3, MBD2 , MBP, MCAM, Mcl-1, MCM, MCM5 , MCMP2, MDH1, MDH2, ME1, Meprin alpha, Metallothionein, Metavinculin, MFAP4, MFI2, mGLUR5, MGP, Mimecan, MINPP1, MLCK, MMR, MMRN1, MN1, Moesin, MP1, MPCA, Myeloperoxidase, MRP 1, MSH6, mTOR, MUCDHL, Multimerin 2, MyBPC3, MYH2, MYH6, MYH7, MYHC, MYL12B , MYL3, MYO5A, Myoferlin , Myosin 18B, Myotrophin, NABC1, NAGLU, NAP1L1 , NAPRT1, NASP , NCAM2 , Nebulin, Nectin-1, Nectin-3, NEDD8, Neogenin, Nesprin2, Neurabin 1, N-Cadherin, Neurofibromin, Neurogranin, Neuropeptide B, Neuropilin-1, Neurotrimin, NF-M, Nidogen-2, NIT2, NME3, nNOS, Noelin, Non-muscle Actin, Non-muscle Myosin IIA, Notch-2, Notch-2 ICD, NPAS3, NPM1, NQO2, NT5C3, Nucleobindin 1, NUP98, OBCAM, OIT3, Olfactomedin-2, Ornithine Carbamoyltransferase , Orosomucoid 2, ORP150, OSBP1, OSCAR, Oncostatin M R beta, Osteoadherin, Oxytocin-neurophysin 1, p16 ARC , P20Sb3, p23, p39 , P4HB, p73, PA2G4, PABP, PACS1, PARVB, PCBP1, PCBP2, PCCA, PCDH7, PCDX8, PCK2 , PCMT1, PCNA, PCPE-1, Proprotein Convertase 9, PCYOX1, PDE1B, PDIA6, PDLIM1, PDLIM5, PDZD2, PEBP4, PEPD, PER1, Perilipin-3, Perilipin-1 , Periostin, Peroxiredoxin 2, Peroxiredoxin 3, Peroxiredoxin 5, PF4V1, PGAM1, PGAM2, PGD, PGDF, PGK-1, PGLS-C-t, PGM1, PGRPL, PHAP1, Phosphoserine Aminotransferase, PI3-Kinase C2 beta, pIgR, PIK3IP1, PIN, PISD, PKLR, PLA2G1B, Plakophilin 1, Plastin L, PLC-gamma 1 , Pleckstrin, Plectin, Plexin B1, Plexin B2, PLOD1, PLOD2, PLS3, Plxdc2, PNP, POR, PPCS, PPOX , PPP2R1B , PPP2R4, PRCP, PRDM13, PRDX 1, PRELP, PREP, PRG2, Prion protein PrP , Profilin 1, Properdin, Prosaposin, Prostaglandin D Synthase, Proteasome 20S a+b, Proteasome 20S alpha, Proteasome 20S alpha 5, Proteasome 20S b7, Proteasome 26S S5, Proteasome beta 1, Proteasome subunit alpha type 6, Proteasome subunit beta type 2, Proteasome subunit beta type 4, Coagulation Factor XIV, Protein Z, Prouroguanylin, PRSS23, PRSS3, Myeloblastin, PSMA1, PSMA2, PSMA4, PSMA7, PSMB5, PSMC3 , PSMD1, PSMD9 , PTEN, PTK 7, PTMA, PTP gamma, PTP kappa, PTP mu, PTPRS, PTPRZ, PYGL, PZP, QDPR, QPRT, Quiescin Q6, Rab7a, Ran, RanGAP1, RAP1AB, Rbm15, RCL , Reg1A, Reg3A, RHOC, RhoGDI, Ribonuclease A, Ribonuclease Inhibitor, Ribonuclease T2, RKIP, RNA Polymerase II, RNASE4, RNASE6, RPL10, RPL10A , RPL11, RPL12, RPL14 , RPL17, RPL22, RPL5, RPL7A, RPLP0 , RPS10, RPS11, RPS12, RPS19, RPS2, RPS20 , RPS23, RPS25, RPS28, RPS3, RPS5, RREB1, RSU1, S100 A1, S100 A11, S100 A7, S100 A9, S100P, SAA, SAA4a, Salivary alpha amylase, SAMSN1, SBP-1, SBSN, SDF4, SDNSF, SDPR, Secretogranin V, Semaphorin 6B, Semaphorin 7A, Semenogelin I, Semenogelin II, Serpin A11, Serpin A7, Serpin B3, Serpin B6, Serpin B8, Serpin F2, Serpin A10, Serpin B1, Serpin B4, Serpin E2, SerRS, SET, SEZ6L2, SF20, SH3BGRL, SH3BGRL3, SHANK1, SHC1, SHIP, SHMT1, SHP-1, Siglec-1, Siglec-14, SIM2 , SIRP beta 1, Six3, SLC38A10, SLITRK1, SLURP1, SMA, SMC4 , SMPD4, SOD1, SOD2, SOD3, SOD4, Somatostatin, SORD, SorLA, SOX4, SP-D, Spectrin beta-5, SPEN, SPINK7, SPTBN1, Src(1), SREC-II, STAT3, Stathmin 1, Sterol carrier protein 2, STI1, STOM, SUCLG1, SUMO3, Symplekin, SynCAM, Syntaxin 7, TAB182, TAGLN2, Talin1, Talin1&2, TAX1BP3 , TBCA, TCEB2, Tcf20, TCN1, TCP1 eta, Tenascin C, Tenascin X(1), TFF2(1), TGM3, Thioredoxin-1, Thimet Oligopeptidase, Thymosin b10, Titin, TLS, TMEM223, TOB2 , TOP2B, TPM4, Tripeptidyl-peptidase I, Transaldolase 1, Transketolase, Transthyretin, TRAP1, TRAP220, TRF 2, Triosephosphate isomerase, Tropomyosin 3, TRP-1, TRPS1, Trypsinogeb-2, TrypsinPan, Tryptophanyl, TSR2, TUBA6, TWF2, TXNDC4, TXNDC5, TXNRD2, UBE2D3, Ube2L3, UBE2N, Ubiquitin+1, UCH-L1, UFM 1, UGGT, UNC-13 Homolog D, UNC45A, UNC5H4, UPB1 , UQCRB, UQCRH , URB, URB2, UROC1, UROD, URP2, USP14 , USP5 , Uteroglobin, Utrophin, VAP-1, VAP-A, VCP, VDAC1 , Versican isoform V0, Vimentin B, VNN1, VSIG4, WDR1, WISP2, WNK2, YB1 , YY1, ZBTB4, ZC3H4-N-t, ZC3H8, ZDHHC18 , ZNF671, Zyxin

Upon receipt, all components of the Human Glycosylation Array L4 kit should be stored at -20°C. Once thawed, the glass slide and Cy3 equivalent dye-conjugated Streptavidin should be kept at –20°C and all other components may be stored at 4°C. The entire kit should be used within 6 months of purchase.