大港澳53040论坛-免费完整资料

规格

规格	8样本、50样本(产品加服务)、8样本(产品加服务)、22样本(产品加服务)、22样本、50样本
样本类型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
检测方法	Fluorescence Laser Scanner
定量与否	Quantitative
载体	Glass Slide
适用种属	Human
检测靶标	IGF-1, VEGF-A, Galectin-3, HGF, RAGE, Leptin, TGF beta 1, EGF, IGF-2, Alpha-fetoprotein, CA125, CA15-3, CA19-9, Cathepsin L, CEA, CEACAM-1, DKK-1, ErbB2, FABP3, GDF-15, GLP-1, Glucagon, hCG beta, Midkine, NSE, PAPP-A, Presenilin 1, Prolactin, PYY, Thyroglobulin, uPA, CA72-4, GLP-1 Degraded, SCF / KITLG, TROP1 / EpCAM, MIP-3 alpha / CCL20, Pentraxin-3 / TSG-14, GOLM1 / GP73, Osteopontin / SPP1, Serpin B3 / SCCA1
靶标数	40

详细描述

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
Alpha-fetoprotein Gene Symbol: AFP HPAFP Gene ID: 174 Accession #: P02771 ELISA Kit: RayBio Human AFP ELISA	CA125 Gene Symbol: AFP HPAFP Gene ID: 174 Accession #: P02771 ELISA Kit: RayBio Human AFP ELISA	CA15-3 Gene Symbol: MUC16 CA125 Gene ID: 94025 Accession #: Q8WXI7 ELISA Kit: RayBio Human CA-125 ELISA	CA19-9 Gene Symbol: MUC1 PUM Gene ID: 4582 Accession #: P15941 ELISA Kit: RayBio Human CA15-3 ELISA	CA72-4 Accession #: ELISA Kit: RayBio Human CA19-9 ELISA
Cathepsin L Accession #: ELISA Kit: RayBio Custom Human CA72-4 ELISA	CEA Gene Symbol: CTSL CTSL1 Gene ID: 1514 Accession #: P07711 ELISA Kit: RayBio Human Cathepsin L ELISA	CEACAM-1 Gene Symbol: CEACAM5 CEA Gene ID: 1048 Accession #: P06731 ELISA Kit: RayBio Human CEA ELISA	DKK-1 Gene Symbol: CEACAM1 BGP BGP1 Gene ID: 634 Accession #: P13688 ELISA Kit: RayBio Human CEACAM-1 ELISA	EGF Gene Symbol: DKK1 UNQ492/PRO1008 Gene ID: 22943 Accession #: O94907 ELISA Kit: RayBio Human Dkk-1 ELISA
TROP1 (EpCAM) Gene Symbol: EGF Gene ID: 1950 Accession #: P01133 ELISA Kit: RayBio Human EGF ELISA	ErbB2 Gene Symbol: EPCAM GA733-2 M1S2 M4S1 MIC18 TACSTD1 TROP1 Gene ID: 4072 Accession #: P16422 ELISA Kit: RayBio Human TROP1 ELISA	FABP3 Gene Symbol: ERBB2 HER2 MLN19 NEU NGL Gene ID: 2064 Accession #: P04626 ELISA Kit: RayBio Human ErbB2 ELISA	Galectin-3 Gene Symbol: FABP3 FABP11 MDGI Gene ID: 2170 Accession #: P05413 ELISA Kit: RayBio Human FABP-3 ELISA	GDF-15 Gene Symbol: LGALS3 MAC2 Gene ID: 3958 Accession #: P17931 ELISA Kit: RayBio Human Galectin-3 ELISA
GLP-1 Gene Symbol: GDF15 MIC1 PDF PLAB PTGFB Gene ID: 9518 Accession #: Q99988 ELISA Kit: RayBio Human GDF-15 ELISA	GLP-1 Degraded Gene Symbol: GCG Gene ID: 2641 Accession #: P01275 ELISA Kit: RayBio Human GLP-1 (1-37a) ELISA	Glucagon Accession #: ELISA Kit: RayBio	GOLM1 (GP73) Gene Symbol: GOLM1 C9orf155 GOLPH2 PSEC0242 UNQ686/PRO1326 Gene ID: 51280 Accession #: Q8NBJ4 ELISA Kit: RayBio Human GOLM1/GP-73 ELISA	hCG beta Gene Symbol: CGB CGB3 CGB5 CGB7 CGB8 Gene ID: 1082 93659 94027 94115 Accession #: P01233 ELISA Kit: RayBio Human hCG-beta ELISA
HGF Gene Symbol: HGF HPTA Gene ID: 3082 Accession #: P14210 ELISA Kit: RayBio Human HGF ELISA	IGF-1 Gene Symbol: IGF1 IBP1 Gene ID: 3479 Accession #: P05019 ELISA Kit: RayBio Human IGF-1 ELISA	IGF-2 Gene Symbol: IGF2 PP1446 Gene ID: 3481 Accession #: P01344 ELISA Kit: RayBio	Leptin Gene Symbol: LEP OB OBS Gene ID: 3952 Accession #: P41159 ELISA Kit: RayBio Human Leptin ELISA	Midkine Gene Symbol: MDK MK1 NEGF2 Gene ID: 4192 Accession #: P21741 ELISA Kit: RayBio Human MDK ELISA
MIP-3 alpha (CCL20) Gene Symbol: CCL20 LARC MIP3A SCYA20 Gene ID: 6364 Accession #: P78556 ELISA Kit: RayBio Human MIP-3 alpha ELISA	NSE Gene Symbol: ENO2 Gene ID: 2026 Accession #: P09104 ELISA Kit: RayBio	Osteopontin (SPP1) Gene Symbol: SPP1 BNSP OPN PSEC0156 Gene ID: 6696 Accession #: P10451 ELISA Kit: RayBio Human Osteopontin (OPN) ELISA	PAPP-A Gene Symbol: PAPPA Gene ID: 5069 Accession #: Q13219 ELISA Kit: RayBio Human PAPP-A ELISA	Pentraxin-3 (TSG-14) Gene Symbol: PTX3 TNFAIP5 TSG14 Gene ID: 5806 Accession #: P26022 ELISA Kit: RayBio Human PTX-3 ELISA
Presenilin 1 Gene Symbol: PSEN1 AD3 PS1 PSNL1 Gene ID: 5663 Accession #: P49768 ELISA Kit: RayBio Human Presenilin 1 ELISA	Prolactin Gene Symbol: PRL Gene ID: 5617 Accession #: P01236 ELISA Kit: RayBio	PYY Gene Symbol: PYY Gene ID: 5697 Accession #: P10082 ELISA Kit: RayBio Human PYY ELISA	RAGE Gene Symbol: MOK RAGE RAGE1 Gene ID: 5891 Accession #: Q9UQ07 ELISA Kit: RayBio Human RAGE ELISA	SERPINB3 (SCCA1) Gene Symbol: SERPINB3 SCCA SCCA1 Gene ID: 6317 Accession #: P29508 ELISA Kit: RayBio Human Serpin B3/SCCA1 ELISA
SCF Gene Symbol: KITLG MGF SCF Gene ID: 4254 Accession #: P21583 ELISA Kit: RayBio Human SCF ELISA	TGF beta 1 Gene Symbol: TGFB1 TGFB Gene ID: 7040 Accession #: P01137 ELISA Kit: RayBio Human TGF-beta 1 ELISA	Thyroglobulin Gene Symbol: TG Gene ID: 7038 Accession #: P01266 ELISA Kit: RayBio Human Thyroglobulin ELISA	uPA Gene Symbol: PRAP1 UPA UNQ608/PRO1195 Gene ID: 118471 Accession #: Q96NZ9 ELISA Kit: RayBio Human uPA ELISA	VEGF-A Gene Symbol: VEGFA VEGF Gene ID: 7422 Accession #: P15692 ELISA Kit: RayBio Human VEGF-A ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Human Cancer Discovery Array Q2 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
30 ml Centrifuge Tube
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.