大港澳53040论坛-免费完整资料

规格

规格	2 样本、4 样本
样本类型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
检测方法	Chemiluminescence
定量与否	Semi-Quantitative
载体	Membrane
适用种属	Human
检测靶标	Cystatin C, Resistin, Trappin-2, ACE-2, PAI-1, Albumin, Clusterin, FABP1, AMPKa1, p53, Caspase-3, Caspase-8, cIAP-2, Cytochrome C, HSP27, HSP60, HSP70, HTRA2, IGFBP-5, Livin, p21, SMAC, XIAP, p27, A1BG, ACK1, ACPP, ACTH, ADAM-9, ADAMTS-10, ADAMTS-13, ADAMTS-15, ADAMTS-17, ADAMTS-18, ADAMTS-19, ADAMTS-4, ADAMTS-5, ADAMTSL-2, Afamin, Aldolase A, Aldolase B, Aldolase C, ALK, Alpha Lactalbumin, Alpha-fetoprotein, ALPP, AMICA, Amylin, Annexin A7, APC, APCS, Apelin, ASPH, Attractin, B3GNT1, BAF57, BCAM, Beta Defensin 4, beta IG-H3, Biglycan, BMP-9, BMX, BNIP2, BNP, BTK, c-Abl, C-Peptide, C3a, CA125, CA15-3, CA19-9, CA9, Cadherin-13, Calbindin D, Calcitonin, Calreticulin, CART, Cathepsin D, Cathepsin L, Cathepsin S, CBP, CCK, CD200, CD23, CD24, CD38, CD45, CD46, CD47, CD59, CD61, CD71, CD74, CD79 alpha, CD97, CEA, CEACAM-1, Ceruloplasmin, CFHR2, Chemerin, CHI3L1, Chymase, CK-MB, Claudin-3, Claudin-4, COCO, Complement Factor H, Contactin-1, CPN2, Creatinine, CRTAM, CSH1, CutA, Cyclin D1, Cystatin A, Cystatin B, D-Dimer, DBI, Desmin, DLL1, DLL4, DMP-1, E-Cadherin, Endothelin Receptor A, Enolase 2, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, ERRa, Erythropoietin R, ESAM, EV15L, EXTL2, FABP2, FABP3, FAK, FAP, Fen 1, FER, Ferritin, Fetuin A, Fetuin B, FGFR1, FGFR1 alpha, FGFR2, Fibrinogen, Fibrinopeptide A, Fibronectin, Ficolin-3, FIH, FOLR1, FOXN3, FOXO1, FoxP3, FRK, Furin, FYN, GADD45A, Galanin, Galectin-1, Galectin-3BP, Galectin-7, gamma-Thrombin, Gas 1, Gastrin, GATA-3, GATA-4, Gelsolin, Ghrelin, GLP-1, GMNN, GPBB, GPI, GPR-39, GPX1, GPX3, GRP, GRP78, GSR, GST, HADHA, HAI-1, HAI-2, Haptoglobin, hCG alpha, Hck, Hemopexin, Hepcidin, HOXA10, HSP10, HSP20, HSP40, HSP90, HSPA8, IBSP, IL-34, IMP1, INSL3, INSRR, Integrin alpha-5, ITK, ITM2B, Kallikrein 10, Kallikrein 11, Kallikrein 14, Kallikrein 2, Kallikrein 5, Kallikrein 6, Kallikrein 7, Kallikrein 8, KCC3, KCTD10, KIF3B, KLF4, LAG-3, Layilin, LDL R, Legumain, LH, LIMPII, LIN41, LOX-1, LPS, LRG1, LTK, Lumican, Lyn, LYRIC, LYVE-1, LZTS1, Mammaglobin A, Marapsin, MATK, MBL, Mer, Mesothelin, MICB, Midkine, MINA, MSHa, MTUS1, Myoglobin, NAIP, Nanog, NELL2, Neprilysin, Nestin, NET1, Netrin G2, Netrin-1, Netrin-4, Neurokinin-A, Neuropeptide Y, NF1, NM23-H1/H2, Notch-1, NPTX1, NPTXR, NR3C3, Omentin, Osteocalcin, P-Cadherin, PAK7, Pancreastatin, PCAF, PD-1, PGRP-S, PI 16, PIM2, PKM2, Plasminogen, Podocalyxin, POMC, PON1, PON2, PPARg2, PPP2R5C, Presenilin 1, Presenilin 2, Pro-BDNF, Pro-MMP-13, Pro-MMP-7, Pro-MMP-9, Procalcitonin, Progesterone, Prohibitin, ProSAAS, Prostasin, Protein p65, PSA-free, PSA-total, PSP, PTH, PTHLP, PTPRD, PYK2, PYY, Ras, RBP4, RECK, RELM alpha, Ret, RIP1, ROCK1, ROCK2, ROR1, ROR2, Ryk, S100 A6, S100 A8, SART1, SART3, SCG3, Selenoprotein P, SEMA3A, Serotonin, Serpin A12, Serpin A4, Serpin A9, Serpin B5, Serpin D1, Serpin G1, Serpin I1, SERTAD2, SHBG, SNCG, SOST, SOX17, SOX2, SPINK1, SRMS, SSEA-1, SSEA-4, SSTR2, SSTR5, SYK, Syndecan-1, Syndecan-3, TAF4, TEC, TFF1, Thrombomodulin, Thymidine Kinase-1, Thyroglobulin, TNK1, TOPORS, TPA, TPM1, TRA-1-60, TRA-1-81, Transferrin, TRKB, Troponin C, Troponin I, Troponin T, TRPC1, TRPC6, TRPM7, TSH, TSLP, TXK, TYK2, TYRO10, Uromodulin, Vasopressin, VDUP-1, VGF, VIP Receptor 2, Vitamin D Receptor, Vitamin K-dependent protein S, Vitronectin, VWF, Wilms Tumor 1, ZAG, ZAP70, 11b-HSD1, Alpha 1 AG, Alpha 1 Microglobulin, APN, APP, ARB1, BAI-1, Beta 2M, C1qTNF1, C2, C5/C5a, C7, C8b, C9, CBG, Chromogranin A, CLEC14A, Cytokeratin18, Cytokeratin19, DEFA1/3, Endorphin Beta, ENPP2, Factor XIII B, Fc gamma RIIIB, Fcg RIIB/C, GRP75, hCGb, IDUA, IL-28B, LTF, Nesfatin, OCT3/4, Pappalysin-1, PEPSINOGEN I, PEPSINOGEN II, PI 3Kinase p85 alpha, pro-Glucagon, ROS, S-100b, S100A4, VEGF R1, Vitamin D-BP, SPARC-Like 1, Cathepsin B / Pro-Cathepsin B, IL-33 / IL-1F11, Angiopoietin-like protein 3, Angiopoietin-like protein 4, Apolipoprotein A-I / ApoA1, Apolipoprotein A-II / ApoA2, Apolipoprotein A-IV / ApoA4, Apolipoprotein B / ApoB, Apolipoprotein B-100 / Apo B-100, Apolipoprotein C-I / ApoC1, Apolipoprotein C-II / ApoC2, Apolipoprotein C-III / ApoC3, Apolipoprotein D / ApoD, Apolipoprotein E / ApoE, Apolipoprotein E-III / ApoE3, Apolipoprotein H / ApoH, BLAME / SLAMF8, CK beta 8-1 / CCL23, Coagulation Factor II / Thrombin / F2, HSP32 / HO-1 / HMOX1, PDX-1 / IPF1, CD90 / Thy1, Adipsin / Complement Factor D, Angiotensinogen / Serpin A8, CD44 / Heparan Sulfate Proteoglycan, CLEC3B / Tetranectin, Carnosine Dipeptidase-1 / CNDP1, Contactin-2 / TAG1, SCGF / CLEC11a, CD36 / SR-B3, TROP1 / EpCAM, Serpin A1 / Alpha-1-Antitrypsin, Serpin F1 / PEDF, 2B4 / CD244, ACE / CD143, APE1 / APEX1, Carboxypeptidase A1 / CPA1, CD26 / DPPIV, CD55 / DAF, Calsyntenin-1 / Alcadein-alpha, COX-2 / PTGS2, CRP / C-Reactive Protein, DCBLD2 / ESDN, FABP4 / A-FABP, HE4 / WFDC2, Cytokeratin 8 / KRT8, Osteopontin / SPP1, OX40 / TNFRSF4, Park7 / DJ-1, Pancreatic polypeptide / ProPPY / PP, Pleiotrophin / PTN, Serpin A3 / Alpha-1-Antichymotrypsin, Serpin A5 / Protein C Inhibitor, ADAM-17 / TACE, TFF3 / Trefoil Factor 3, TIM-1 / KIM-1, 4-1BB / TNFRSF9 / CD137, Trypsin 1 / PRSS1, Visfatin / PBEF1, ADAMTS-1 / METH1, Alpha-2 Microglobulin, BAFF / TNFSF13B, Survivin / BIRC5, Apolipoprotein M / ApoM
靶标数	493

详细描述

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

11b-HSD1, 2B4, 4-1BB, A1BG, A2M, ABL1, ACE, ACE-2, ACK1, ACPP, ACTH, ADAM-9, ADAMTS-1, ADAMTS-10, ADAMTS-13, ADAMTS-15, ADAMTS-17, ADAMTS-18, ADAMTS-19, ADAMTS-4 , ADAMTS-5, ADAMTS-L2, Adipsin, Afamin, AFP, ALBUMIN, Aldolase A, Aldolase B, Aldolase C, ALK, Alpha 1 AG, Alpha 1 Microglobulin, Alpha Lactalbumin, ALPP, AMICA, AMPKa1, Amylin, ANGPTL3, ANGPTL4, Annexin A7, APC, APCS, Apelin, Apex1, APN, ApoA1, ApoA2, ApoA4, ApoB, ApoB100, ApoC1, ApoC2, ApoC3, ApoD, ApoE, ApoE3, ApoH, ApoM, APP, ARB1, ASPH, Attractin, B3GNT1, BAF57, BAFF, BAI-1, BCAM, Beta 2M, Beta Defensin 4, Beta IG-H3, Biglycan, BLAME, BMP-9, BMX, BNIP2, BNP, Btk, C1qTNF1, C2, C3a, C5/C5a, C7, C8b, C9, CA125, CA15-3, CA19-9, CA9, Cadherin-13, Calbindin D, Calcitonin, Calreticulin, Calsyntenin-1, CART, Caspase-3, Caspase-8, Cathepsin B, Cathepsin D, Cathepsin L, Cathepsin S, CBG, CBP, CCK, CD200, CD23, CD24, CD36, CD38, CD44, CD45, CD46, CD47, CD55, CD59, CD61, CD71, CD74, CD79 alpha, CD90, CD97, CEA, CEACAM-1, Ceruloplasmin, CFHR2, Chemerin, CHI3L1, Chromogranin A, Chymase, cIAP-2, Ck beta 8-1, CK-MB, Claudin-3, Claudin-4, CLEC14A, CLEC3B, Clusterin, CNDP1, COCO, Complement factor H, Contactin-1, Contactin-2, COX-2, CPA1, C-peptide, CPN2, Creatinine, CRP, CRTAM, CSH1, CutA, Cyclin D1, Cystatin A, Cystatin B, Cystatin C, Cytochrome C, Cytokeratin 8, Cytokeratin18, Cytokeratin19, DBI, DCBLD2, D-Dimer, DEFA1/3, Desmin, DLL1, DLL4, DMP-1, DPPIV, E-Cadherin, Endorphin Beta, Endothelin Receptor A, Enolase 2, ENPP2, EpCAM, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, ERRa, Erythropoietin R, ESAM, EV15L, EXTL2, FABP1, FABP2, FABP3, FABP4, Factor XIII B, FAK, FAP, Fc gamma RIIIB, Fcg RIIB/C, Fen 1, FER, Ferritin, Fetuin A, Fetuin B, FGFR1, FGFR1 alpha, FGFR2, Fibrinogen, Fibrinopeptide A, Fibronectin, Ficolin-3, FIH, FOLR1, FOXN3, FoxO1, FoxP3, FRK, Furin, Fyn, GADD45A, Galanin, Galectin-1, Galectin-3BP, Galectin-7, gamma-Thrombin, Gas1, Gastrin, GATA-3, GATA-4, Gelsolin, Ghrelin, GLP-1, GMNN, GPBB, GPI, GPR-39, GPX1, GPX3, GRP, GRP75, GRP78, GSR, GST, HADHA, HAI-1, HAI-2, Haptoglobin, hCG alpha, hCGb, Hck, HE4, Hemopexin, Hepcidin, HOXA10, HSP10, HSP20, HSP27, HSP32, HSP40, HSP60, HSP70, HSP90, HSPA8, HTRA2, IBSP, IDUA, IGF2BP1, IGFBP-5, IL-28B, IL-33, IL-34, INSL3, INSRR, Integrin alpha V, Itk, ITM2B, Kallikrein 10, Kallikrein 11, Kallikrein 14, Kallikrein 2, Kallikrein 5, Kallikrein 6, Kallikrein 7, Kallikrein 8, KCC3, KCTD10, KIF3B, KLF4, LAG-3, Layilin, LDL R, Legumain, LH, LIMPII, LIN41, Livin, LOX-1, LPS, LRG1, LTF, LTK, Lumican, Lyn, LYRIC, LYVE-1, LZTS1, Mammaglobin A, Marapsin, MATK, MBL, Mer, Mesothelin, MICB, Midkine, MINA, MSHa, MTUS1, Myoglobin, NAIP, Nanog, NELL2, Neprilysin, Nesfatin, Nestin, NET1, Netrin G2, Netrin-4, Neurokinin-A, Neuropeptide Y, NF1, NM23-H1/H2, Notch-1, NPTX1, NPTXR, NR3C3, Ntn1, OCT3/4, Omentin, Osteocalcin, Osteopontin, OX40, p21, p27, p53, PAI-1, PAK7, Pancreastatin, Pancreatic Polypeptide, Pappalysin-1, PARK7, P-Cadherin, PCAF, PD-1, PDX-1, PEDF, PEPSINOGEN I, PEPSINOGEN II, PGRP-S, PI 16, PI 3Kinase p85 alpha, PIM2, PKM2, Plasminogen, Podocalyxin, POMC, PON1, PON2, PPARg2, PPP2R5C, Presenilin 1, Presenilin 2, Pro-BDNF, Procalcitonin, Pro-Cathepsin B, Progesterone, pro-Glucagon, Prohibitin, Pro-MMP-13, Pro-MMP-7, Pro-MMP-9, ProSAAS, Prostasin, Protein p65, PSA-Free, PSA-total, PSP, PTH, PTHLP, PTN, PTPRD, PYK2, PYY, Ras, RBP4, RECK, RELM alpha, Resistin, RET, RIP1, ROCK1, ROCK2, ROR1, ROR2, ROS, RYK, S100A4, S100A6, S100A8, S-100b, SART1, SART3, SCG3, SCGF, Selenoprotein P, SEMA3A, Serotonin, Serpin A1, Serpin A12, Serpin A3, Serpin A4, Serpin A5, Serpin A8, Serpin A9, Serpin B5, Serpin D1, Serpin G1, Serpin I1, SERTAD2, SHBG, SMAC, SNCG, SOST, SOX17, SOX2, SPARCL1, SPINK1, SRMS, SSEA-1, SSEA-4, SSTR2, SSTR5, Survivin, SYK, Syndecan-1, Syndecan-3, TACE, TAF4, Tec, TFF1, TFF3, Thrombin, Thrombomodulin, Thymidine Kinase-1, Thyroglobulin, TIM-1, TNK1, TOPORS, TPA, TPM1, TRA-1-60, TRA-1-81, Transferrin, Trappin-2, TRKB, Troponin C, Troponin I, Troponin T, TRPC1, TRPC6, TRPM7, Trypsin 1, TSH, TSLP, TXK, Tyk2, TYRO10, Uromodulin, Vasopressin, VDUP-1, VEGF R1, VGF, VIP Receptor 2, Visfatin, Vitamin D Receptor, Vitamin D-BP, Vitamin K-dependent protein S, Vitronectin, VWF, Wilms Tumor 1, XIAP, ZAG, ZAP70

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Tyrosine Phosphorylation Array C2 Membranes
Blocking Buffer
Biotinylated Anti-Phosphotyrosine Antibody
1,000X HRP-Streptavidin Concentrate
20X Wash Buffer I Concentrate
20X Wash Buffer II Concentrate
2X Cell Lysis Buffer Concentrate
Detection Buffer C
Detection Buffer D
Incubation Tray w/ Lid
Protease Inhibitor Cocktail
Phosphatase Inhibitor Cocktail II
Plastic Sheets
Array Map Template
User Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or plastic Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system:
- CCD Camera
- X-Ray Film and a suitable film processor
- Gel documentation system
- Or another chemiluminescent detection system capable of imaging a western blot

Protocol Outline

Blocking
Sample Incubation
First Wash
Biotinylated Antibody Cocktail Incubation
Second Wash
HRP-Streptavidin Incubation
Third Wash
Chemiluminescence Detection

Storage/Stability

Store kit at ≤-20°C immediately upon arrival. Kit must be used within the 6 month expiration date.