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规格

规格	8 样本、4 样本
样本类型	Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
检测方法	Fluorescence Laser Scanner
定量与否	Semi-Quantitative
载体	Glass Slide
适用种属	Human
检测靶标	Cystatin C, Resistin, Trappin-2, 4-1BB, ACE-2, Adipsin, ANGPTL4, CRP, PAI-1, Albumin, Clusterin, FABP1, Osteopontin, TFF3, TIM-1, AMPKa1, p53, Caspase-3, Caspase-8, cIAP-2, Cytochrome C, HSP27, HSP60, HSP70, HTRA2, IGFBP-5, Livin, p21, SMAC, Survivin, XIAP, p27, 2B4, A1BG, A2M, ABL1, ACE, ACK1, ACPP, ACTH, ADAM-9, ADAMTS-1, ADAMTS-10, ADAMTS-13, ADAMTS-15, ADAMTS-17, ADAMTS-18, ADAMTS-19, ADAMTS-4, ADAMTS-5, Afamin, Aldolase A, Aldolase B, Aldolase C, ALK, Alpha Lactalbumin, ALPP, AMICA, Amylin, ANGPTL3, Annexin A7, APC, APCS, Apelin, APEX1, ApoA1, ApoA2, ApoA4, ApoB, ApoB100, ApoC1, ApoC2, ApoC3, ApoD, ApoE, ApoE3, ApoH, ApoM, ASPH, Attractin, B3GNT1, BAF57, BAFF, BCAM, Beta Defensin 4, beta IG-H3, Biglycan, BLAME, BMP-9, BMX, BNIP2, BNP, BTK, C-Peptide, C3a, CA125, CA15-3, CA19-9, CA9, Cadherin-13, Calbindin D, Calcitonin, Calreticulin, Calsyntenin-1, CART, Cathepsin B, Cathepsin D, Cathepsin L, Cathepsin S, CBP, CCK, CD200, CD23, CD24, CD36, CD38, CD44, CD45, CD46, CD47, CD55, CD59, CD61, CD71, CD74, CD79 alpha, CD90, CD97, CEA, CEACAM-1, Ceruloplasmin, CFHR2, Chemerin, CHI3L1, Chymase, Ck beta 8-1, CK-MB, Claudin-3, Claudin-4, CLEC3B, CNDP1, COCO, Complement Factor H, Contactin-1, Contactin-2, COX-2, CPN2, Creatinine, CRTAM, CSH1, CutA, Cyclin D1, Cystatin A, Cystatin B, Cytokeratin 8, D-Dimer, DBI, DCBLD2, Desmin, DLL1, DLL4, DMP-1, DPPIV, E-Cadherin, Endothelin Receptor A, Enolase 2, EpCAM, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, ERRa, Erythropoietin R, ESAM, EV15L, EXTL2, FABP2, FABP3, FABP4, FAK, FAP, Fen 1, FER, Ferritin, Fetuin A, Fetuin B, FGFR1, FGFR1 alpha, FGFR2, Fibrinogen, Fibrinopeptide A, Fibronectin, Ficolin-3, FIH, FOLR1, FOXN3, FOXO1, FoxP3, FRK, Furin, FYN, GADD45A, Galanin, Galectin-1, Galectin-3BP, Galectin-7, gamma-Thrombin, Gastrin, GATA-3, GATA-4, Gelsolin, Ghrelin, GLP-1, GMNN, GPBB, GPI, GPR-39, GPX1, GPX3, GRP, GRP78, GSR, GST, HADHA, HAI-1, HAI-2, Haptoglobin, hCG alpha, Hck, HE4, Hemopexin, Hepcidin, HOXA10, HSP10, HSP20, HSP32, HSP40, HSP90, HSPA8, IBSP, IGF2BP1, IL-33, IL-34, INSL3, INSRR, ITK, ITM2B, Kallikrein 10, Kallikrein 11, Kallikrein 14, Kallikrein 2, Kallikrein 5, Kallikrein 6, Kallikrein 7, Kallikrein 8, KCC3, KCTD10, KIF3B, KLF4, LAG-3, Layilin, LDL R, Legumain, LH, LIMPII, LIN41, LOX-1, LPS, LRG1, LTK, Lumican, Lyn, LYRIC, LYVE-1, LZTS1, Mammaglobin A, Marapsin, MATK, MBL, Mer, Mesothelin, MICB, Midkine, MINA, MSHa, MTUS1, Myoglobin, NAIP, Nanog, NELL2, Neprilysin, Nestin, NET1, Netrin G2, Netrin-4, Neurokinin-A, Neuropeptide Y, NF1, NM23-H1/H2, Notch-1, NPTX1, NPTXR, NR3C3, Omentin, Osteocalcin, OX40, P-Cadherin, PAK7, Pancreastatin, Pancreatic Polypeptide, Park7, PCAF, PD-1, PDX-1, PEDF, PGRP-S, PI 16, PIM2, PKM2, Plasminogen, Podocalyxin, POMC, PON1, PON2, PPARg2, PPP2R5C, Presenilin 1, Presenilin 2, Pro-BDNF, Pro-Cathepsin B, Pro-MMP-13, Pro-MMP-7, Pro-MMP-9, Procalcitonin, Progesterone, Prohibitin, ProSAAS, Prostasin, Protein p65, PSA-free, PSA-total, PSP, PTH, PTHLP, PTN, PTPRD, PYK2, PYY, Ras, RBP4, RECK, RELM alpha, Ret, RIP1, ROCK1, ROCK2, ROR1, ROR2, Ryk, SART1, SART3, SCG3, Selenoprotein P, SEMA3A, Serotonin, Serpin A1, Serpin A12, Serpin A3, Serpin A4, Serpin A5, Serpin A8, Serpin A9, Serpin B5, Serpin D1, Serpin G1, Serpin I1, SERTAD2, SHBG, SNCG, SOST, SOX17, SOX2, SPARCL1, SPINK1, SRMS, SSEA-1, SSEA-4, SSTR2, SSTR5, SYK, Syndecan-1, Syndecan-3, TACE, TAF4, TEC, TFF1, Thrombin, Thrombomodulin, Thymidine Kinase-1, Thyroglobulin, TNK1, TOPORS, TPA, TPM1, TRA-1-60, TRA-1-81, Transferrin, TRKB, Troponin C, Troponin I, Troponin T, TRPC1, TRPC6, TRPM7, Trypsin 1, TSH, TSLP, TXK, TYK2, TYRO10, Uromodulin, Vasopressin, VDUP-1, VGF, VIP Receptor 2, Visfatin, Vitamin D Receptor, Vitamin K-dependent protein S, Vitronectin, VWF, Wilms Tumor 1, ZAG, ZAP70, 11b-HSD1, ADAMTS-L2, AFP, Alpha 1 AG, Alpha 1 Microglobulin, APN, APP, ARB1, BAI-1, Beta 2M, C1qTNF1, C2, C5/C5a, C7, C8b, C9, CBG, Chromogranin A, CLEC14A, CPA1, Cytokeratin18, Cytokeratin19, DEFA1/3, Endorphin Beta, ENPP2, Factor XIII B, Fc gamma RIIIB, Fcg RIIB/C, Gas1, GRP75, hCGb, IDUA, IL-28B, Integrin alpha V, LTF, Nesfatin, Ntn1, OCT3/4, Pappalysin-1, PEPSINOGEN I, PEPSINOGEN II, PI 3Kinase p85 alpha, pro-Glucagon, ROS, S-100b, S100A4, S100A6, S100A8, SCGF, VEGF R1, Vitamin D-BP
靶标数	493

详细描述

Introduction

Protein acetylation plays an unusually prominent role in cell signaling, development and growth. The RayBio® G-Series Human Protein Acetylation Antibody Array 2 is a very rapid, convenient, and sensitive assay that can simultaneously detect multiple protein acetylation of lysine and be used to monitor the activation or function of important biological pathways.

RayBiotech is committed to develop a series of acetylation antibody arrays. This multiplex array is specifically designed for simultaneous identification of the relative levels of acetylation of lysine of 493 different Human Proteins in cell lysate. By monitoring the changes in protein acetylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort performing an analysis of immunoprecipitation and/or Western Blot.

To use the Human Protein Acetylation Antibody Array G2, treated or untreated cell lysate is added into antibody array glass slide wells. The antibody array slide wells are washed, and a biotinylated anti- acetylated-lysine antibody is then used to detect the acetylated-lysine on target proteins. After incubation with a fluorescent dye-conjugated streptavidin (Cy3 equivalent), the slides can then be imaged using a laser scanner, such as the Axon GenePix, using the Cy3 channel.

Product Features

High sensitivity and specificity
Low sample volume (10-100 µl per array)
Large dynamic range of detection
Compatible with most sample types
Test 4 or 8 samples on each slide
Suitable for high-throughput assays

Target Names

11b-HSD1, 2B4, 4-1BB, A1BG, A2M, ABL1, ACE, ACE-2, ACK1, ACPP, ACTH, ADAM-9, ADAMTS-1, ADAMTS-10, ADAMTS-13, ADAMTS-15, ADAMTS-17, ADAMTS-18, ADAMTS-19, ADAMTS-4 , ADAMTS-5, ADAMTS-L2, Adipsin, Afamin, AFP, ALBUMIN, Aldolase A, Aldolase B, Aldolase C, ALK, Alpha 1 AG, Alpha 1 Microglobulin, Alpha Lactalbumin, ALPP, AMICA, AMPKa1, Amylin, ANGPTL3, ANGPTL4, Annexin A7, APC, APCS, Apelin, Apex1, APN, ApoA1, ApoA2, ApoA4, ApoB, ApoB100, ApoC1, ApoC2, ApoC3, ApoD, ApoE, ApoE3, ApoH, ApoM, APP, ASPH, Attractin, B3GNT1, BAF57, BAFF, BAI-1, BCAM, Beta 2M, Beta Defensin 4, Beta IG-H3, Biglycan, BLAME, BMP-9, BMX, BNIP2, BNP, Btk, C2, C3a, C5/C5a, C7, C8B, C9, CA125, CA15-3, CA19-9, CA9, Cadherin-13, Calbindin, Calbindin D, Calcitonin, Calreticulin, Calsyntenin-1, CART, Caspase-3, Caspase-8, Cathepsin B, Cathepsin D, Cathepsin L, Cathepsin S, CBP, CCK, CD200, CD23, CD24, CD36, CD38, CD44, CD45, CD46, CD47, CD55, CD59, CD61, CD71, CD74, CD79 alpha, CD90, CD97, CEA, CEACAM-1, Ceruloplasmin, CFHR2, Chemerin, CHI3L1, Chromogranin A, Chymase, cIAP-2, Ck beta 8-1, CK-MB, Claudin-3, Claudin-4, CLEC3B, Clusterin, CNDP1, COCO, Complement factor H, Contactin-1, Contactin-2, Corticosteroid-binding globulin, COX-2, C-peptide, CPN2, Creatinine, CRP, CRTAM, CSH1, cTnT / Troponin T, CutA, Cyclin D1, Cystatin A, Cystatin B, Cystatin C, Cytochrome C, Cytokeratin 8, Cytokeratin18, Cytokeratin19, DBI, DCBLD2, D-Dimer, DEFA1/3, Defensin, Desmin, DLL1, DLL4, DMP-1, DPPIV, E-Cadherin, Endorphin Beta, Endothelin Receptor A, Enolase 2, ENPP2, EpCAM, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, ERRa, Erythropoietin R, ESAM, EV15L, EXTL2, FABP1, FABP2, FABP3, FABP4, Factor XIII A, Factor XIII B, FAK, FAP, Fc RIIB/C, Fen 1, FER, Ferritin, Fetuin A, Fetuin B, FGFR1, FGFR1 alpha, FGFR2, Fibrinogen, Fibrinopeptide A, Fibronectin, Ficolin-3, FIH, FOLR1, FOXN3, FoxO1, FoxP3, FRK, FSH, Furin, Fyn, GADD45A, Galanin, Galectin-1, Galectin-3BP, Galectin-7, gamma-Thrombin, Gas1, Gastrin, GATA-3, GATA-4, Gelsolin, Ghrelin, GLP-1, GMNN, GPBB, GPI, GPR-39, GPX1, GPX3, GRP, GRP75, GRP78, GSR, GST, HADHA, HAI-1, HAI-2, Haptoglobin, hCG alpha, hCGb, Hck, HE4, Hemopexin, Hepcidin, HOXA10, HSP10, HSP20, HSP27, HSP32, HSP40, HSP60, HSP70, HSP90, HSPA8, HTRA2, IBSP, IGF2BP1, IGFBP-5, IL-23p19, IL-33, IL-34, IL36RN, INSL3, INSRR, Integrin alpha V, Itk, ITM2B, Kallikrein 10, Kallikrein 11, Kallikrein 14, Kallikrein 2, Kallikrein 5, Kallikrein 6, Kallikrein 7, Kallikrein 8, KCC3, KCTD10, KIF3B, KLF4, LAG-3, Layilin, LDL R, Legumain, LH, LIMPII, LIN41, Livin, LOX-1, LPS, LRG1, LTF, LTK, Lumican, Lyn, LYRIC, LYVE-1, LZTS1, Mammaglobin A, Marapsin, MATK, MBL, MBL-2, Mer, Mesothelin, MICB, Midkine, MINA, MSHa, MTUS1, Myoglobin, NAIP, Nanog, NELL2, NEP, Nesfatin, Nestin, NET1, Netrin G2, Netrin-4, Neurokinin-A, Neuropeptide Y, NF1, NM23-H1/H2, Notch-1, NPTX1, NPTXR, NR3C3, Ntn1, OCT3/4, Omentin, Osteocalcin, Osteopontin, OX40, p21, p27, p53, PAI-1, PAK7, Pancreastatin, Pancreatic Polypeptide, Pappalysin-1, PARK7, P-Cadherin, PCAF, PD-1, PDX-1, PEDF , PEPSINOGEN I, PEPSINOGEN II, PGRP-S, PI 16, PI 3Kinase p85 beta, PIM2, PKM2, Plasminogen, Podocalyxin, POMC, PON1, PON2, PPARg2, PPP2R5C, Presenilin 1, Presenilin 2, Pro-BDNF, Procalcitonin, Pro-Cathepsin B, Progesterone, pro-Glucagon, Prohibitin, Pro-MMP-13, Pro-MMP-7, Pro-MMP-9, ProSAAS, Prostasin, Protein p65, PSA-Free, PSA-total, PSP, PTH, PTHLP, PTN, PTPRD, PYK2, PYY, Ras, RBP4, RECK, RELM alpha, Resistin, RET, RIP1, ROCK1, ROCK2, ROR1, ROR2, ROS, RYK, S100A4, S100A6, S100A8, S-100b, SART1, SART3, SCG3, Selenoprotein P, SEMA3A, Serotonin, Serpin A1, Serpin A12, Serpin A3, Serpin A4, Serpin A5, Serpin A8, Serpin A9, Serpin B5, Serpin D1, Serpin I1, SERTAD2, SHBG, SMAC, SNCG, Somatotropin, SOST, SOX17, SOX2, SPARCL1, SPINK1, SRMS, SSEA-1, SSEA-4, SSTR2, SSTR5, Survivin, SYK, Syndecan-1, Syndecan-3, TACE, TAF4, Tec, TFF1, TFF3, Thrombin, Thrombomodulin, Thymidine Kinase-1, Thyroglobulin, TIM-1, TNK1, TOPORS, TPA, TPM1, TRA-1-60, TRA-1-81, Transferrin, Trappin-2, TRKB, Troponin C, Troponin I, TRPC1, TRPC6, TRPM7, Trypsin 1, TSH, TSLP, TXK, Tyk2, TYRO10, Uromodulin, Vasopressin, VDUP-1, VEGF R1, VGF, VIP Receptor 2, Visfatin, Vitamin D Receptor, Vitamin D-BP, Vitamin K-dependent protein S, Vitronectin, VWF, Wilms Tumor 1, XIAP, ZAG, ZAP70

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

RayBio® Glass Slide(s)
Blocking Buffer
Biotinylated Anti-Acetylated-Lysine Antibody
Cy3 equivalent-Conjugated Streptavidin
20X Wash Buffer I Concentrate
20X Wash Buffer II Concentrate
Wash Buffer III
2X Cell Lysis Buffer Concentrate
Protease Inhibitor Cocktail
Adhesive film

Other Materials Required

Shaker
Laser scanner for fluorescence detection
Aluminum foil
Distilled water
Plastic box
50 ml Centrifuge tube
Isopropanol (2-propanol)

Protocol Outline

Dry the Glass Slide
Blocking and Incubation
Fluorescence Detection

Storage/Stability

Upon receipt, the kit should be stored at ≤-20°C. Please use within 6 months from the date of shipment.