大港澳53040论坛-免费完整资料

规格

规格	4 样本、8 样本
样本类型	Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
检测方法	Fluorescence Laser Scanner
定量与否	Semi-Quantitative
载体	Glass Slide
适用种属	Human
检测靶标	IFN-gamma, IL-1 alpha, IL-1 beta, IL-8, MCP-1, MIG, MIP-1 beta, RANTES, TNF alpha, Angiopoietin-1, bFGF, CD40 Ligand, Decorin, GASP-1, IFN-beta, IGF-1, IL-10, IL-13, IL-15, IL-17A, IL-2, IL-21, IL-36 Ra, IL-4, IP-10, LIF, NCAM-1, VEGF-A, CCL4, CXCL10, CXCL9, IL-1 F1, IL-1 F5, GRO alpha, EGFR, ErbB3, Erythropoietin, FGF-7, Galectin-3, GM-CSF, HGF, HGFR, IGFBP-2, IL-1 Ra, IL-12 p40, IL-6, MMP-8, PDGF-BB, RAGE, SCF, TGF alpha, TNF RI, CXCL8, IL-1 F2, IL-1 F3, CCL2, Fas, IL-5, CCL5, IL-16, Pentraxin-3, TSG-14, ACRP30, Adiponectin, AgRP, Angiopoietin-2, Apo-1, CCL16, CCL18, CCL25, CCL7, CXCL5, ENA-78, FGF-6, Growth Hormone, HCC-4, IGF-1 R, IGFBP-1, IGFBP-3, IL-1 R1, IL-1 R4, IL-11, IL-12 p70, IL-6 R, Insulin, Leptin, Leptin R, Lymphotactin, M-CSF, MARC, MCP-3, MIF, Oncostatin M, Osteoprotegerin, PARC, PDGF-AA, PDGF-AB, SAA, Serum Amyloid A, Somatotropin, ST2, TECK, TGF beta 1, TIMP-1, TIMP-2, TNF RII, TNFRSF11B, TNFRSF1A, TNFRSF1B, TNFRSF27, TNFRSF6, XCL1, XEDAR, CD106, CXCL16, GPNMB, Lipocalin-2, NGAL, Osteoactivin, VCAM-1, Angiogenin, Angiostatin, CCL1, CCL13, CD31, CXCL11, EGF, Endostatin, GCSF, GRO alpha/beta/gamma, I-309, I-TAC, KDR, MCP-4, MMP-1, MMP-9, PECAM-1, PLGF, TCA-3, Thrombopoietin, Tie-2, TPO, uPAR, VEGF-D, VEGFR2, VEGFR3, bax, CD40, DR4, DR5, DR6, Fas Ligand, IGF-2, IGFBP-4, IGFBP-6, TNF beta, TNFRSF10A, TNFRSF10B, TNFRSF10C, TNFRSF10D, TNFRSF21, TNFRSF5, TNFSF1B, TNFSF5, TNFSF6, TRAIL R1, TRAIL R2, TRAIL R3, TRAIL R4, CCL11, CCL20, CD54, Eotaxin-1, GDNF, IL-1 R2, MIP-3 alpha, TGF beta 2, TGF beta 3, 6Ckine, AcPL, Activin A, Activin B, Activin C, Activin R1A, Activin R1B, Activin R2A, Activin R2A/B, AK155, ALCAM, ALK-1, ALK-2, ALK-3, ALK-4, ALK-5, ALK-6, Alpha Chain and Full Length, Amphiregulin, Angiopoietin-3, Angiopoietin-4, ANGPTL1, ANGPTL2, ANGPTL7, Apelin Receptor, APJ, APRIL, Artemin, AXL, B7-1, BACE-1, BAFF R, BCMA, BD-1, BDNF, beta-Catenin, beta-NGF, Betacellulin, BIK, BLC, BLR-1, BMP-11, BMP-15, BMP-2, BMP-3, BMP-3b, BMP-4, BMP-5, BMP-6, BMP-7, BMP-8, BMPER, BMPR-IA, BMPR-IB, BMPR-II, BRAK, BTC, Cardiotrophin-1, CCL14, CCL15, CCL17, CCL19, CCL21, CCL22, CCL24, CCL26, CCL27, CCL28, CCL3, CCN2, CCN3, CCN4, CCR1, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CD102, CD105, CD114, CD117, CD11b, CD122, CD127, CD14, CD152, CD163, CD166, CD170, CD220, CD27, CD30, CD30 Ligand, CD50, CD56, CD62L, CD80, CD91 Cluster II, CDH5, Cerberus 1, CF10, Chem R23, Chordin-Like 2, Chordin-Like-1, CHRDL1, CLC, CNTF, CNTF R alpha, Coagulation Factor III, Coagulation Factor X, Common gamma Chain, CRAM-A/B, CRIM1, Cripto-1, Crossveinless-2, CRTH-2, Cryptic, CSFR1, CSK, CT-1, CTACK, CTGF, CTLA-4, CV-2, CX3CL1, CXCL1, CXCL12, CXCL13, CXCL14, CXCL2, CXCL4, CXCL6, CXCL7, CXCR1, CXCR2, CXCR3, CXCR4, CXCR5, CXCR6, D6, DAN, DANCE, DcR3, DKK-1, Dkk-3, Dkk-4, DkkL1, DLK-1, DR3, Dtk, E-Selectin, EDA-A2, EDAR, EDG-1, EG-VEGF, EMAP-II, EML2, EN-RAGE, Endocan, Endoglin, Endothelin-1, Eotaxin-2, Eotaxin-3, Epiregulin, ErbB1, ErbB2, ErbB4, FA1, FACX, FADD, FAM3B, FGF R3, FGF R4, FGF R5, FGF-10, FGF-11, FGF-12, FGF-13 1B, FGF-16, FGF-17, FGF-18, FGF-19, FGF-20, FGF-21, FGF-23, FGF-4, FGF-5, FGF-8, FGF-9, FGF-BP, FGFRL1, FIL1 delta, FIL1 epsilon, FIL1 eta, FIL1 zeta, FLRG, Flt-3 Ligand, Follistatin, Follistatin-like 1, Fractalkine, Frizzled-1, Frizzled-3, Frizzled-4, Frizzled-5, Frizzled-6, Frizzled-7, Fusin, GASP-2, GCP-2, GCSF R, GDF-1, GDF-10, GDF-11, GDF-15, GDF-3, GDF-5, GDF-8, GDF-9, GDF-9B, GDNF R, GFR alpha-1, GFR alpha-2, GFR alpha-3, GFR alpha-4, GITR, GITR Ligand, Glucagon, Glut1, Glut2, Glut3, Glut5, Glyoxalase 1, Glypican 3, Glypican 5, GM-CSF R alpha, gp130, Granzyme A, Grb2, Gremlin-1, Growth Hormone R, HB-EGF, HCC-1, HCR, Hepassocin, HER4, HFREP-1, HRG1-alpha, HRG1-beta 1, HVEM, ICAM-1, ICAM-2, ICAM-3, ICAM-5, IDE, IFN-alpha/beta R1, IFN-alpha/beta R2, IFN-gamma R1, IFN-lambda 1, IFN-lambda 2, IGF-2 R, IGFBP-7, IGFBP-rp1, IL-1 F10, IL-1 F6, IL-1 F7, IL-1 F8, IL-1 F9, IL-1 H1, IL-1 HY2, IL-1 R Acp, IL-1 R3, IL-1 R5, IL-1 R6, IL-1 R8, IL-1 R9, IL-1 RAPL2, IL-1 Rrp2, IL-10 R alpha, IL-10 R beta, IL-12 R beta 1, IL-12 R beta 2, IL-13 R alpha 1, IL-13 R alpha 2, IL-13 R1, IL-15 R alpha, IL-17 RA, IL-17 RB, IL-17 RC, IL-17 RD, IL-17B, IL-17C, IL-17D, IL-17E, IL-17F, IL-18 BP alpha, IL-18 R alpha, IL-18 R beta, IL-19, IL-2 R alpha, IL-2 R beta, IL-2 R gamma, IL-20, IL-20 R alpha, IL-20 R beta, IL-21 R, IL-22, IL-22 R alpha 1, IL-22BP, IL-22Ra2, IL-23, IL-23 R, IL-24, IL-25, IL-26, IL-27, IL-27 R alpha, IL-28A, IL-29, IL-3, IL-3 R alpha, IL-31, IL-31 RA, IL-36 alpha, IL-36 beta, IL-36 gamma, IL-37, IL-38, IL-4 R, IL-5 R alpha, IL-7, IL-7 R alpha, IL-8 RA, IL-8 RB, IL-9, IL1RAPL1, INHBC, Insulin R, Insulysin, Integrin alpha-M, ITGAL, KGF, KGF-2, Kininogen, Kininostatin, KITLG, Kremen-1, Kremen-2, L-Selectin, LAP2, LBP, LCK, LECT2, Lefty-A, LFA-1 alpha, LIF R alpha, LIGHT, Lipocalin-1, LRP-1 Cluster II, LRP-6, LTBP1, Lymphotoxin beta, Lymphotoxin beta R, M-CSF R, MAC-1, MADH9, MDC, MEC, MET, MFG-E8, MFRP, MICA, MIP-1 alpha, MIP-1 delta, MIP-2, MIP-3 beta, MMP-10, MMP-11, MMP-12, MMP-13, MMP-14, MMP-15, MMP-16, MMP-19, MMP-2, MMP-20, MMP-24, MMP-25, MMP-3, MMP-7, MPIF-2, MSP alpha, MT3-MMP, MT5-MMP, MT6-MMP, MuSK, Myostatin, NAP-2, Neuritin, NeuroD1, Neuropilin-2, Neurturin, NGFR, Nidogen-1, NOV, Nr-CAM, NRG1 Isoform GGF2, NRG1-alpha, NRG1-beta 1, NRG2, NRG3, NT-3, NT-4, Orexin-A, Orexin-B, Osteocrin, Osteonectin, OX40 Ligand, P-Selectin, PD-ECGF, PDGF R alpha, PDGF R beta, PDGF-C, PDGF-D, Peroxiredoxin-6, Persephin, PK1, Platelet Factor 4, PLUNC, Prdx6, Pref-1, Progranulin, Prolactin, RANK, RELM beta, RELT, ROBO4, S100 A10, S100 A12, S100 A8/A9, SCF R, SDF-1, sFRP-1, sFRP-3, sFRP-4, sgp130, Shh-N, SIGIRR, Siglec-5, Siglec-9, SLPI, SMAD1, SMAD4, SMAD5, SMAD7, SMAD8, SMAD9, Soggy-1, Sonic Hedgehog N-Terminal, SPARC, Spinesin, TACI, TARC, TCCR, TCK-1, TFPI, TGF beta R2, TGF beta R3, TGF-beta 5, TGF-beta RI, Thromboplastin, Thrombospondin-1, Thrombospondin-2, Thrombospondin-4, Thymidine Phosphorylase, Thymopoietin isform alpha, Thyroid Peroxidase, Tie-1, TIMP-3, TIMP-4, Tissue Factor, TL1A, TLR1, TLR2, TLR3, TLR4, TMEFF1, TMEFF2, TMPRSS5, TNF RIII, TNFRSF11A, TNFRSF12A, TNFRSF13B, TNFRSF13C, TNFRSF14, TNFRSF16, TNFRSF17, TNFRSF18, TNFRSF19, TNFRSF19L, TNFRSF25, TNFRSF3, TNFRSF6B, TNFRSF7, TNFRSF8, TNFSF10, TNFSF11, TNFSF12, TNFSF14, TNFSF15, TNFSF18, TNFSF3, TNFSF4, TNFSF8, Tomoregulin-1, TP, TPX, TRADD, TRAIL, TRANCE, TREM-1, TROY, TSG-6, TSLP R, TWEAK, TWEAK R, TYMP, TYRO3, Ubiquitin+1, uPA, Vasorin, VE-Cadherin, VEGF-B, VEGF-C, WFIKKN, WFIKKNRP, WIF-1, WISP-1, WSX-1
靶标数	507

详细描述

Introduction

Protein acetylation plays an unusually prominent role in cell signaling, development and growth. The RayBio® G-Series Human Protein Acetylation Antibody Array 1 is a very rapid, convenient, and sensitive assay that can simultaneously detect multiple protein acetylation of lysine and be used to monitor the activation or function of important biological pathways.

RayBiotech is committed to develop a series of acetylation antibody arrays. This multiplex array is specifically designed for simultaneous identification of the relative levels of acetylation of lysine of 507 different Human Proteins in cell lysate. By monitoring the changes in protein acetylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort performing an analysis of immunoprecipitation and/or Western Blot.

To use the Human Protein Acetylation Antibody Array G1, treated or untreated cell lysate is added into antibody array glass slide wells. The antibody array slide wells are washed, and a biotinylated anti- acetylated-lysine antibody is then used to detect the acetylated-lysine on target proteins. After incubation with a fluorescent dye-conjugated streptavidin (Cy3 equivalent), the slides can then be imaged using a laser scanner, such as the Axon GenePix, using the Cy3 channel.

Product Features

High sensitivity and specificity
Low sample volume (10-100 µl per array)
Large dynamic range of detection
Compatible with most sample types
Test 4 or 8 samples on each slide
Suitable for high-throughput assays

Target Names

6Ckine, Activin A, Activin B, Activin C, Activin RIA (ALK-2), Activin RIB (ALK-4), Activin RII A/B, Activin RIIA, Adiponectin (Acrp30), AgRP, ALCAM, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiopoietin-4, Angiopoietin-like 1, Angiopoietin-like 2, Angiopoietin-like Factor, Angiostatin, APJ, APRIL, AR (Amphiregulin), Artemin, Axl, B7-1 (CD80), BAFF R (TNFRSF13C), BCMA (TNFRSF17), BD-1, BDNF, beta-Catenin, BAX, beta-NGF, BIK, BLC (BCA-1/CXCL13), BMP-2, BMP-3, BMP-3b (GDF-10), BMP-4, BMP-5, BMP-6, BMP-7, BMP-8, BMP-15, BMPR-IA (ALK-3), BMPR-IB (ALK-6), BMPR-II, BTC, Cardiotrophin-1 (CT-1), CCL14 (HCC-1/HCC-3), CCL28 (VIC), CCR1, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CD14, CD27 (TNFRSF7), CD30 (TNFRSF8), CD30 Ligand (TNFSF8), CD40 (TNFRSF5), CD40 Ligand (TNFSF5/CD154), CD 163, Cerberus 1, Chem R23, Chordin-Like 1, Chordin-Like 2, CLC, CNTF, CNTF R alpha, Coagulation Factor III (Tissue Factor), CRIM 1, Cripto-1, CRTH-2, Cryptic, Csk, CTACK (CCL27), CTGF (CCN2), CTLA-4 (CD152), CV-2 (Crossveinless-2), CXCL14 (BRAK), CXCL16, CXCR1 (IL-8 RA), CXCR2 (IL-8 RB), CXCR3, CXCR4 (fusin), CXCR5 (BLR-1), CXCR6, D6, DAN, DANCE, DcR3 (TNFRSF6B), Decorin, Dkk-1, Dkk-3, Dkk-4, DR3 (TNFRSF25), DR6 (TNFRSF21), Dtk, EDA-A2, EDAR, EDG-1, EGF, EGF R (ErbB1), EG-VEGF (PK1), EMAP-II, ENA-78, Endocan, Endoglin (CD105), Endostatin, Endothelin, EN-RAGE, Eotaxin (CCL11), Eotaxin-2 (MPIF-2), Eotaxin-3 (CCL26), Epiregulin, ErbB2, ErbB3, ErbB4, Erythropoietin, E-Selectin, FADD, FAM3B, Fas (TNFRSF6), Fas Ligand, FGF Basic, FGF-BP, FGF R3, FGF R4, FGF R5, FGF-4, FGF-5, FGF-6, FGF-7 (KGF), FGF-8, FGF-9, FGF-10 (KGF-2), FGF-11, FGF-12, FGF-13 1B, FGF-16, FGF-17, FGF-18, FGF-19, FGF-20, FGF-21, FGF-23, FLRG, Flt-3 Ligand, Follistatin, Follistatin-like 1, Fractalkine, Frizzled-1, Frizzled-3, Frizzled-4, Frizzled-5, Frizzled-6, Frizzled-7, Galectin-3, GASP-1 (WFIKKNRP), GASP-2 (WFIKKN), GCP-2 (CXCL6), GCSF, G-CSF R (CD 114), GDF1, GDF3, GDF5, GDF8, GDF9, GDF11, GDF-15, GDNF, GFR alpha-1, GFR alpha-2, GFR alpha-3, GFR alpha-4, GITR (TNFRF18), GITR Ligand (TNFSF18), Glucagon, Glut1, Glut2, Glut3, Glut5, Glypican 3, Glypican 5, GM-CSF, GM-CSF R alpha, Granzyme A, GREMLIN, GRO, GRO-a, Growth Hormone (GH), Growth Hormone R (GHR), HB-EGF, HCC-4 (CCL16), HCR (CRAM-A/B), Hepassocin, GLO-1, HGF, HGFR, HRG-alpha, HRG-beta 1, HVEM (TNFRSF14), I-309, ICAM-1, ICAM-2, ICAM-3 (CD50), ICAM-5, IFN-alpha/beta R1, IFN-alpha/beta R2, IFN-beta, IFN-gamma, IFN-gamma R1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGFBP-rp1 (IGFBP-7), IGF-I, IGF-I SR, IGF-II, IGF-II R, IL-1 alpha, IL-1 beta, IL-1 F5 (FIL1delta), IL-1 F6 (FIL1 epsilon), IL-1 F7 (FIL1 zeta), IL-1 F8 (FIL1 eta), IL-1 F9 (IL-1 H1), IL-1 F10 (IL-1HY2), IL-1 R3 (IL-1 R AcP), IL-1 R4 (ST2), IL-1 R6 (IL-1 Rrp2), IL-1 R8, IL-1 R9, IL-1 ra, IL-1 sRI, IL-1 sRII, IL-2, IL-2 R alpha, IL-2 R beta (CD122), IL-2 R gamma, IL-3, IL-3 R alpha, IL-4, IL-4 R, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-7 R alpha, IL-8, IL-9, IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-12 R beta 1, IL-12 R beta 2, IL-13, IL-13 R alpha 1, IL-13 R alpha 2, IL-15, IL-15 R alpha, IL-16, IL-17, IL-17B, IL-17B R, IL-17C, IL-17D, IL-17E, IL-17F, IL-17R, IL-17RC, IL-17RD, IL-18 BPa, IL-18 R alpha (IL-1 R5), IL-18 R beta (AcPL), IL-19, IL-20, IL-20 R alpha, IL-20 R beta, IL-21, IL-21 R, IL-22, IL-22 BP, IL-22 R, IL-23, IL-23 R, IL-24, IL-26, IL-27, IL-28A, IL-29, IL-31, IL-31 RA, BACE-1, FACX, Insulin, Insulin R, Insulysin (IDE), IP-10, I-TAC (CXCL11), Kininostatin (kininogen), Kremen-1, Kremen-2, Latent TGF-beta bp1, LBP, Lck, LECT2, Lefty - A, Leptin (OB), Leptin R, LFA-1 alpha, LIF, LIF R alpha, LIGHT (TNFSF14), Lipocalin-1, Lipocalin-2, LRP-1, LRP-6, L-Selectin (CD62L), Lymphotactin (XCL1), Lymphotoxin beta (TNFSF3), Lymphotoxin beta R (TNFRSF3), MAC-1, MCP-1, MCP-2, MCP-3, MCP-4 (CCL13), M-CSF, M-CSF R, MDC, MFG-E8, MFRP, MICA, MIF, MIG, MIP-1a, MIP-1b, MIP-1d, MIP 2, MIP-3 alpha, MIP-3 beta, MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11 (Stromelysin-3), MMP-12, MMP-13, MMP-14, MMP-15, MMP-16 (MT3-MMP), MMP-19, MMP-20, MMP-24 (MT5-MMP), MMP-25 (MT6-MMP), MSP alpha Chain, Musk, NAP-2, NCAM-1 (CD56), Neuritin, NeuroD1, Neuropilin-2, Neurturin, NGF R, Nidgen-1, NOV (CCN3), NrCAM, NRG1 Isoform GGF2, NRG2, NRG3, NT-3, NT-4, Orexin A, Orexin B, OSM, Osteoactivin (GPNMB), Osteocrin, Osteoprotegerin (TNFRSF11B), OX40 Ligand (TNFSF4), PARC (CCL18), PD-ECGF, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-C, PDGF-D, PECAM-1 (CD31), Pentraxin3 (TSG-14), Persephin, PF4 (CXCL4), PlGF, PLUNC, Pref-1, Progranulin, Prolactin, P-selectin, RAGE, RANK (TNFRSF11A), RANTES, RELM beta, RELT (TNFRSF19L), ROBO4, S100 A8/A9, S100A10, SAA, SCF, SCF R (CD117), SDF-1 (CXCL12), sFRP-1, sFRP-3, sFRP-4, sgp130, SIGIRR, Siglec-5 (CD170), Siglec-9, SLPI, Smad 1, Smad 4, Smad 5, Smad 7, Smad 8, Prdx6, Soggy-1, Sonic Hedgehog (Shh N-terminal), SPARC, Spinesin, TACI (TNFRSF13B), Tarc, TCCR (WSX-1), TECK (CCL25), TFPI, TGF-alpha, TGF-beta 1, TGF-beta 2, TGF-beta 3, TGF-beta 5, TGF-beta RI (ALK-5), TGF-beta RII, Grb2, TGF-beta RIII, Thrombopoietin (TPO), TPX, Thrombospondin-1, Thrombospondin-2, Thrombospondin-4, Thymopoietin, Tie-1, Tie-2, TIMP-1, TIMP-2, TIMP-3, TIMP-4, TL1A (TNFSF15), TLR1, TLR2, TLR3, TLR4, TMEFF1 (Tomoregulin-1), TMEFF2, TNF-alpha, TNF-beta, TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TRADD, TRAIL (TNFSF10), TRAIL R1 (DR4/TNFRSF10A), TRAIL R2 (DR5/TNFRSF10B), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), TRANCE, TREM-1, TROY (TNFRSF19), TSG-6, TSLP R, TWEAK (TNFSF12), TWEAK R (TNFRSF12), Ubiquitin+1, uPA, uPAR, Vasorin, VCAM-1 (CD106), VE-Cadherin, VEGF, VEGF R2 (KDR), VEGF R3, VEGF-B, VEGF-C, VEGF-D, VEGI (TNFSF15), WIF-1, WISP-1 (CCN4), XEDAR

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

RayBio® Glass Slide(s)
Blocking Buffer
Biotinylated Anti-Acetylated-Lysine Antibody
Cy3 equivalent-Conjugated Streptavidin
20X Wash Buffer I Concentrate
20X Wash Buffer II Concentrate
Wash Buffer III
2X Cell Lysis Buffer Concentrate
Protease Inhibitor Cocktail
Adhesive film

Other Materials Required

Shaker
Laser scanner for fluorescence detection
Aluminum foil
Distilled water
Plastic box
50 ml Centrifuge tube
Isopropanol (2-propanol)

Protocol Outline

Dry the Glass Slide
Blocking and Incubation
Fluorescence Detection

Storage/Stability

Upon receipt, the kit should be stored at ≤-20°C. Please use within 6 months from the date of shipment.