大港澳53040论坛-免费完整资料

规格

规格	8样本、16样本
样本类型	Plasma, Serum, Hydrothorax
检测方法	Fluorescence Laser Scanner
定量与否	Semi-Quantitative
载体	Glass Slide
适用种属	Human

详细描述

Product Feature

Small sample volume required (2 µl)
Detects antibody specificity
Detects autoantibody expression
Affordable, quick and simple to use

Target Name

Scroll over each spotted protein for more information
14-3-3 theta Gene Symbol: YWHAQ Gene ID: 10971 Accession #: P27348	Annexin A1 Gene Symbol: ANXA1 ANX1 LPC1 Gene ID: 301 Accession #: P04083 ELISA Kit: RayBio Custom Human Annexin A1 ELISA	Annexin A2 Gene Symbol: ANXA2 ANX2 ANX2L4 CAL1H LPC2D Gene ID: 302 Accession #: P07355	CAGE Gene Symbol: DDX53 CAGE Gene ID: 168400 Accession #: Q86TM3	Cathepsin D Gene Symbol: CTSD CPSD Gene ID: 1509 Accession #: P07339 ELISA Kit: RayBio Custom Human Cathepsin D ELISA
CDK2 Gene Symbol: CDK2 CDKN2 Gene ID: 1017 Accession #: P24941	c-Myc (MYC) Gene Symbol: MYC BHLHE39 Gene ID: 4609 Accession #: P01106 ELISA Kit: RayBio Custom Human c-Myc ELISA	Cyclin A Gene Symbol: CCNA2 CCN1 CCNA Gene ID: 890 Accession #: P20248	Cyclin B1 Gene Symbol: CCNB1 CCNB Gene ID: 891 Accession #: P14635	Cyclin D1 Gene Symbol: CCND1 Accession #: Q6FI00
DKK-1 Gene Symbol: DKK1 UNQ492/PRO1008 Gene ID: 22943 Accession #: O94907 ELISA Kit: RayBio Human Dkk-1 ELISA	GBU4-5 Gene Symbol: TDRD12 Gene ID: 25044 Accession #: Q587J7	HSP70-9B/HSPA9/GRP-75 Gene Symbol: HSPA9 GRP75 HSPA9B mt-HSP70 Gene ID: 3313 Accession #: P38646	Hu-D Gene Symbol: ELAVL4 HUD PNEM Gene ID: 1996 Accession #: P26378	IMDH2 Gene Symbol: IMPDH2 IMPD2 Gene ID: 3615 Accession #: P12268
IMP1 Gene Symbol: IGF2BP1 CRDBP VICKZ1 ZBP1 Gene ID: 10642 Accession #: Q9NZI8	IMP2/IGF2BP2/p62 Gene Symbol: IGF2BP2 IMP2 VICKZ2 Gene ID: 10644 Accession #: Q9Y6M1	IMP3 Gene Symbol: IGF2BP3 IMP3 KOC1 VICKZ3 Gene ID: 10643 Accession #: O00425	LAMR1 Gene Symbol: RPSA LAMBR LAMR1 Gene ID: 3921 Accession #: P08865	MAGEA4 Gene Symbol: MAGEA4 MAGE4 Gene ID: 4103 Accession #: P43358
MALAT1 Gene Symbol: MALAT1 Gene ID: 29665 Accession #: Q9UHZ2	NY-ESO-1 Gene Symbol: CTAG1A CTAG CTAG1 ESO1 LAGE2 LAGE2A; CTAG1B LAGE2B Gene ID: 1485; 246100 Accession #: P78358	p53 Gene Symbol: TP53 P53 Gene ID: 7157 Accession #: P04637 ELISA Kit: RayBio Human p53 ELISA	Paxillin Gene Symbol: PXN Gene ID: 9718 Accession #: P49023-2	PGAM1 Gene Symbol: PGAM1 PGAMA CDABP0006 Gene ID: 5223 Accession #: P18669
PGP9.5 Gene Symbol: UCHL1 Gene ID: 7345 Accession #: P09936	Recoverin Gene Symbol: RCVRN RCV1 Gene ID: 5957 Accession #: P35243	SOX2 Gene Symbol: SOX2 Gene ID: 6657 Accession #: P48431 ELISA Kit: RayBio Custom Human SOX2 ELISA	Survivin (BIRC5) Gene Symbol: BIRC5 API4 IAP4 Gene ID: 332 Accession #: O15392	Ubiquilin-1 Gene Symbol: UBQLN1 DA41 PLIC1 Gene ID: 29979 Accession #: Q9UMX0

Application Notes

Suggested Application

Lung cancer IgG autoantibody profiling

Kit Components

RayBio^® Human Lung Cancer IgG Autoantibody G1 Array Slide
RayBio^® G series antibody array accessory (including slide incubation chamber assembly, Gasket, Protective cover, Snap-on sides and adhesive film)
Blocking Buffer (8 ml/bottle)
Wash Buffer 1 (30 ml/bottle)
Wash Buffer 2 (30 ml/bottle)
1,000X Biotin-conjugated anti-human IgG (1.5 µl/1 vial)
1,500X Streptavidin-Conjugated Fluor (1 µl/vial)

Other Materials Required

Small plastic boxes or containers
Orbital shaker or oscillating rocker
Pipettors, pipette tips and other common lab consumables
Distilled water
Aluminum foil
Laser scanner for fluorescence detection

Protocol Outline

See Manual for relevant protocols.

Typical Data

The following image is a typical result of the Human Lung Cancer Autoantibody Protein Array. The arrays were incubated with 1:200 diluted serum samples. The results shown in Fig. A indicate no background from the biotin-labeled anti-human IgG antibody or from the fluorescent dye-labeled streptavidin. In Fig. B, a base background level is established from normal serum. This background can be subtracted during data analysis without affecting the results. Lastly, Figures C and D are the results of two individuals diagnosed with lung cancer. Several autoantibodies show clear binding.

Fig 1. Human serum test of RayBio^® Lung Cancer Autoantibody Detection Array. The protein arrays were respectively incubated with either no serum (A) or with diluted serum samples derived from either normal human (B), lung cancer patient A (C), or lung cancer patient B (D). Biotin labeled BSA positive controls are outlined in red. Human IgG positive controls are outlined in white.

Storage/Stability

Upon receipt, all components of the RayBio^® Human Protein Array kit should be stored at -20 ^°C to -80 ^°C until just before the experiment. At -20 ^°C to -80 ^°C the kit will retain complete activity for up to 6 months. Please use within six months of purchase. Once thawed, the array glass slide and Blocking Buffer should be kept at –20 ^°C and all other component should be stored at 4 ^°C Use within three months after reagents have been thawed.